Microbiology
Microbiology is the scientific field that focuses on the study of microorganisms. These microorganisms include bacteria, viruses, fungi, archaea, and some protozoa. Microbiology is a diverse and multidisciplinary field that encompasses various sub-disciplines, each with its own focus and area of research.
Protocols
Please find below our list of protocols for applications in the field of microbiology
Pouring agar plates is a common technique used in microbiology laboratories to prepare solid media for cultivating microorganisms
Preparing a bacterial inoculum is a crucial step in various microbiological experiments, including antimicrobial susceptibility testing, growth studies, and other laboratory analyses.
An agar streak plate is a common microbiological technique used to isolate and obtain pure colonies of microorganisms from a mixed culture.
CFU/mL (Colony-Forming Units per milliliter) is a measure used to quantify the number of viable bacterial cells in a liquid sample.
Optical density (OD) measurement is a common method used to quantify the concentration of cells in a liquid culture.
The Gram stain is a fundamental technique in microbiology used to differentiate bacterial cells into two major groups: Gram-positive and Gram-negative, based on differences in their cell wall structure.
Catalase and oxidase tests are common biochemical tests used in microbiology to identify and differentiate between different bacterial species based on their metabolic characteristics.
A growth curve is a graphical representation of the growth of microorganisms over time. It provides valuable information about the growth kinetics of a bacterial culture and can help analyze various growth phases
Zones of inhibition are areas of bacterial growth inhibition around a substance, such as an antibiotic or an antimicrobial agent, when tested on a culture plate
MIC, or Minimum Inhibitory Concentration, is a fundamental concept in microbiology used to determine the lowest concentration of an antimicrobial agent that inhibits visible growth of a microorganism.
MBC, or Minimum Bactericidal Concentration, is a microbiological concept that refers to the lowest concentration of an antimicrobial agent that kills a specific percentage (usually 99.9%) of bacterial cells in a given sample.
FIC, or Fractional Inhibitory Concentration, is a microbiological concept used to determine the interaction between two antimicrobial agents when used in combination.
The Crystal Violet Biofilm Assay is a widely used method to quantify bacterial biofilm formation in vitro.